APA
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Schlößer, H., Thelen, M., Lechner, A., Wennhold, K., Garcia-Marquez, M., Rothschild, S., … von Bergwelt-Baildon, M. V. (2018). B cells in esophago-gastric adenocarcinoma are highly differentiated, organize in tertiary lymphoid structures and produce tumor-specific antibodies. Oncoimmunology.
Chicago/Turabian
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Schlößer, H., M. Thelen, A. Lechner, K. Wennhold, M. Garcia-Marquez, S. Rothschild, E. Staib, et al. “B Cells in Esophago-Gastric Adenocarcinoma Are Highly Differentiated, Organize in Tertiary Lymphoid Structures and Produce Tumor-Specific Antibodies.” Oncoimmunology (2018).
MLA
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Schlößer, H., et al. “B Cells in Esophago-Gastric Adenocarcinoma Are Highly Differentiated, Organize in Tertiary Lymphoid Structures and Produce Tumor-Specific Antibodies.” Oncoimmunology, 2018.
BibTeX Click to copy
@article{h2018a,
title = {B cells in esophago-gastric adenocarcinoma are highly differentiated, organize in tertiary lymphoid structures and produce tumor-specific antibodies},
year = {2018},
journal = {Oncoimmunology},
author = {Schlößer, H. and Thelen, M. and Lechner, A. and Wennhold, K. and Garcia-Marquez, M. and Rothschild, S. and Staib, E. and Zander, T. and Beutner, D. and Gathof, B. and Gilles, Ramona and Cukuroglu, Engin and Göke, Jonathan and Shimabukuro-Vornhagen, A. and Drebber, U. and Quaas, A. and Bruns, C. and Hölscher, A. and von Bergwelt-Baildon, M. V.}
}
ABSTRACT Tumor-infiltrating lymphocytes (TILs) are correlated to prognosis of several kinds of cancer. Most studies focused on T cells, while the role of tumor-associated B cells (TABs) has only recently gained more attention. TABs contain subpopulations with distinct functions, potentially promoting or inhibiting immune responses. This study provides a detailed analysis of TABs in gastro-esophageal adenocarcinoma (EAC). Flow cytometric analyses of single cell suspensions of tumor samples, mucosa, lymph nodes and peripheral blood mononuclear cells (PBMC) of EAC patients and healthy controls revealed a distinct B cell compartment in cancer patients. B cells were increased in tumor samples and subset-analyses of TILs showed increased proportions of differentiated and activated B cells and an enrichment for follicular T helper cells. Confocal microscopy demonstrated that TABs were mainly organized in tertiary lymphoid structures (TLS), which resemble lymphoid follicles in secondary lymphoid organs. A panel of 34 tumor-associated antigens (TAAs) expressed in EAC was identified based on public databases and TCGA data to analyze tumor-specific B cell responses using a LUMINEXTM bead assay and flow cytometry. Structural analyses of TLS and the detection of tumor-specific antibodies against one or more TAAs in 48.1% of analyzed serum samples underline presence of anti-tumor B cell responses in EAC. Interestingly, B cells were decreased in tumors with expression of Programmed Death Ligand 1 or impaired HLA-I expression. These data demonstrate that anti-tumor B cell responses are an additional and underestimated aspect of EAC. Our results are of immediate translational relevance to emerging immunotherapies.